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KMID : 1134819970260050965
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Journal of the Korean Society of Food Science and Nutrition
1997 Volume.26 No. 5 p.965 ~ p.971
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Antimutagenic Effect and Active Compound Analysis of Kale Juice in Salmonella Assay System
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Lee Sun-Mi
Park Kun-Young
Lee Sook-Hee
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Abstract
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Antimutagenic effects of kale juice on the mutagenicity induced by aflatoxin B₁(AFB₁) and N-methyl-N’-nitro-N-nitrosoguanidine(MNNG) in Salmonella assay system were studied. The freeze dried kale juice significantly reduced the mutagenicity induced by AFB₁ in Salmonella typhimurium TA100 and TA98. However, the kale juice exhibited less inhibitory effect on the mutagenicity induced by MNNG as the concentrations of the juice sample increased. Also, kale juice after dialysis (＞12,000, Mw) appeared to have 42.3~89.5% of inhibitory effects against AFB₁, however, the dialyzate did not show any inhibitory effect against MNNG. After heating, the kale juice exerted no antimutagenic activities on the mutagenicity of AFB₁ and MNNG. To separate and identify the antimutagenic compounds from the kale juice, the dialyzates were further fractionated by using Sepharose CL-6B-200 gel filtration. Fraction number 13 showed the strong antimutagenic activity against AFB₁, and the fraction exhibited positive results of a characterized colour reactions of protein, carbohydrate and phenolic compound. Therefore, one of the possible active compounds from the kale juice was supposed to a glycoprotein (Mw. 270,000) which seemed unstable with heating.
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KEYWORD
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kale juice, Salmonella assay, antimutagenicity, gel filtration
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